LOCUS       40924_43124        4285 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PCR template vector pTF102 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Fukuda T, Ohya Y, Ohta K.
  TITLE     Conditional genomic rearrangement by designed meiotic recombination 
            using VDE (PI-SceI) in yeast
  JOURNAL   Mol. Genet. Genomics 278 (4), 467-478 (2007)
  PUBMED    17587055
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Fukuda T, Ohta K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2007) Tomoyuki Fukuda, RIKEN; 2-1 Hirosawa, Wako, 
            Saitama 351-0198, Japan (E-mail:tfukuda@riken.jp, 
            Tel:81-48-467-9277(ex.5552), Fax:81-48-462-4691)
REFERENCE   3  (bases 1 to 4285)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4285)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2007"; volume: "278"; issue: "4"; pages: "467-478"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2007) Tomoyuki Fukuda, RIKEN"; volume: " 2-1 Hirosawa, Wako,
            Saitama 351-0198, Japan (E-mail:tfukuda@riken.jp, 
            Tel:81-48-467-9277(ex.5552), Fax"; pages: "81-48-462-4691"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..103
                     /note="sequence containing a part of the recognition
                     sequence of VDE"
     CDS             complement(254..1054)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1055..1270)
                     /label=URA3 promoter
     misc_feature    1284..1384
                     /note="sequence containing a part of the recognition
                     sequence of VDE"
     promoter        complement(1414..1432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1439..1455)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1597..2052
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2078..2182
                     /label=AmpR promoter
     CDS             2183..3040
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3214..3802
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4090..4111
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4126..4156
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4164..4180
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4188..4204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4225..4243
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tggtacgaca tgtattccag gtgcttttgg ttgtggtaag accgttatct ctcaatcttt
       61 gtccaagtac tccaattctg acgccattat ctatgtcggg tgctttgcca agctttttct
      121 ttccaatttt ttttttttcg tcattataaa aatcattacg accgagattc ccgggtaata
      181 actgatataa ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata
      241 atacagtttt ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc
      301 tgtaacgttc accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa
      361 taataatgtc agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg
      421 cgtctccctt gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat
      481 ctcttccacc catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg
      541 caatgtcaac agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt
      601 ctgctaacat caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac
      661 cgctaacaat acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta
      721 ttctgtatac acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc
      781 tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc
      841 cctccatgga aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac
      901 ctaatgcttc aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca
      961 agtttgtttg cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag
     1021 tagcagcacg ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt
     1081 ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata
     1141 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga
     1201 ttaccgaatc aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg
     1261 atgaattgaa aagcttggag aaagtgcgga gaaagaggta atgaaatggc agaagtcttg
     1321 atggaattcc cagagttata tactgaaatg agcggtacta aagaaccaat tatgaagcgt
     1381 actactcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacaattcac
     1441 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1501 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1561 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     1621 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     1681 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     1741 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     1801 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1861 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1921 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1981 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2041 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2161 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2221 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2281 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2341 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2401 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2461 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2521 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2581 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2641 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2701 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2761 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2821 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2881 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2941 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3001 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3061 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3121 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3181 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3241 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3301 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3361 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3421 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3481 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3541 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3601 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3661 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3721 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3781 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3841 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3901 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3961 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4021 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4081 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     4141 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     4201 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggagct
     4261 ccaccgcggt ggcggccgct ctaga
//
